Identification of Apigenin and Luteolin in Artemisia annua L. for the Quality Control

Rattana Phadungrakwittaya, Sirikul Chotewuttakorn, Manoon Piwtong, Onusa Thamsermsang, Tawee Laohapand, Pravit Akarasereenont


Objective: To identify active compounds and establish the chemical fingerprint of Artemisia annua L. for the quality control.
Methods: Thin-layer chromatography (TLC) conditions were developed to screen for 2 common flavonoids (apigenin and luteolin). Three mobile phases were used to isolate these flavonoids in 80% ethanolic extract of A. annua.Hexane : ethyl acetate : acetic acid (31:14:5, v/v) and toluene : 1,4-dioxane : acetic acid (90:25:4, v/v) were used in normal phase TLC (NP-TLC), and 5.5% formic acid in water : methanol (50:50, v/v) were used in reverse phase TLC (RP-TLC). Chromatograms were visualized under visible light after spraying with Fast Blue B Salt. Apigenin and luteolin bands were checked by comparing their Rf values and UV-Vis absorption spectra with reference markers.
Results: Apigenin and luteolin were simultaneously detected with good specificity in RP-TLC condition, while only apigenin was detected in NP-TLC condition. Apigenin band intensity was higher than luteolin band intensity in both conditions.
Conclusion: This knowledge can be applied to the development of quality control assessments to ensure product efficacy and consistency.


Artemisia annua L.; TLC fingerprint; apigenin; luteolin

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