17β-Hydroxysteroid Dehydrogenase Activity in Uterine Cervix of Normal Subjects and Carcinoma Patients

Ruchaneekorn W. Kalpravidh, Bunrueang Niyomporn

Abstract


     The ability of the human normal uterine cervix and cervical carcinoma to interconvert estradiol-17β and estrone is due to the activity of 17β-hydroxysteroid dehydrogenase (17β-HSD). Human cervical 800 x g supernatant fractions were analyzed by incubating with 3H-estradiol (20 nM) and NAD (0.25 μmol) in 50 mM Tris buffer at pH 8.0. The incubation was terminated by adding 1 ml of 100% methanol and the supernatants were evaporated in air. The dry residues were resuspended in methanol, and aliquots were chromatographed in silica gel thin layer plates in benzene : ethanol (9 : 1, V : V). The 17β-HSD activity was determined from the amount of estrone formed and expressed as nanomoles of estrone formed/mg protein X h.
     The 17β-HSD activity was higher in normal cervix than in cervical carcinoma. Estradiol-17β added to incabation mixture caused a slight increase in enzyme activity only in cervical carcinoma while it had no effect in normal tissues. Progesterone administration increased 17β-HSD activity in some cases of normal and carcinoma cervix. These results suggest that changes in enzyme concentrations may play a physiologic role in the regulation of tissue levels of estradiol-17β.

 


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